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KMID : 1134820170460010039
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Journal of the Korean Society of Food Science and Nutrition
2017 Volume.46 No. 1 p.39 ~ p.45
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Determination of Glycyrrhizic Acid Content and Anti-Diabetic Effect of Glycyrrhiza uralensis Depending on Cultivation Region
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Jang Da-Eun
Song Jin
Hwang In-Guk
Lee Sang-Hoon
Choe Jeong-Sook
Hwang Kyung-A
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Abstract
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This study investigated the glycyrrhizic acid content and anti-diabetic activities of Glycyrrhiza uralensis (GU) depending on the cultivation region (Jecheon, Youngju, Gokseong, China, and Uzbekistan). Glycyrrhizic acid accuracy recovery and intra- and inter-day precisions (RSD%) of the method were calculated at 99.10∼107.07% and 3.92 and 6.31% for GU samples, respectively, whereas the limits of detection and quantitation were 0.14 and 0.20 μg/mL. Anti-diabetic activity was measured by α-glucosidase and glucose uptake. GU (20 g) was extracted with 70% ethanol at 70°C for 6 h. The Jecheon and Gokseong GU showed good inhibitory activity compared to the control. The Jecheon, Youngju, and Uzbekistan GU ethanol extracts (100 μg/mL) showed glucose uptakes (in C2C12 myotube) of 124.19, 127.18, and 126.92%, respectively, compared to the positive control. In conclusion, these methods were validated for detection of glycyrrhizic acid in GU, and the results indicate that GU might have potential anti-diabetic activities.
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KEYWORD
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Glycyrrhiza uralensis, glycyrrhizic acid, method validation, α-glucosidase, glucose uptake
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